Gene description for PFN1
Gene name profilin 1
Gene symbol PFN1
Other names/aliases OGCP
SLC25A11
Species Sus scrofa
 Experiment description of studies that identified PFN1 in Polarised retinal pigmented epithelial cells

1
Experiment ID916
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

2
Experiment ID917
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

3
Experiment ID919
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - basolateral - Rep 2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

4
Experiment ID920
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28687758   
OrganismSus scrofa
Experiment descriptionDirectional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
AuthorsKlingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.
Journal name Sci Rep
Publication year2017
SamplePolarised retinal pigmented epithelial cells
Sample namePolarised RPE cells - apical - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Density gradient centrifugation
Flotation density1.12 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170046: EV-METRIC:88%, 88%

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